Journal:

Article Title: The Epstein-Barr Virus Immediate-Early Protein BZLF1 Induces Expression of E2F-1 and Other Proteins Involved in Cell Cycle Progression in Primary Keratinocytes and Gastric Carcinoma Cells

doi: 10.1128/JVI.76.24.12543-12552.2002

Figure Lengend Snippet: Representative cellular genes activated by BZLF1 expressiona

Article Snippet: Although BZLF1 was previously shown to induce a G 0 /G 1 block in HeLa cells ( 11 , 12 ), the results of the microarray experiment in telomerase-immortalized human keratinocyte cells suggested that BZLF1 is unlikely to induce a G 0 /G 1 cell cycle block in this cell type and instead may be activating cell cycle progression. table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Gene Fold activation E2F-1 16 DP-1 43 Cyclin A 15 Cyclin E 35 Cdc25A 18 Thymidine kinase 5 Thymidylate kinase 21 Open in a separate window a RNA from telomerase-immortalized human keratinocytes, mock infected or infected with AdLacZ or AdBZLF1, was analyzed by microarray analysis using the Affymetrix GeneChip HuGeneFL Array.

Techniques: Activation Assay

Journal:

Article Title: The Epstein-Barr Virus Immediate-Early Protein BZLF1 Induces Expression of E2F-1 and Other Proteins Involved in Cell Cycle Progression in Primary Keratinocytes and Gastric Carcinoma Cells

doi: 10.1128/JVI.76.24.12543-12552.2002

Figure Lengend Snippet: BZLF1 increases the expression of E2F-1-responsive genes in some cell types. (A) Telomerase-immortalized keratinocytes or normal human fibroblasts were mock infected or infected with the AdLacZ or AdBZLF1 vector. Immunoblot analysis was performed 2 days later to quantitate expression of cyclin E, SLBP, or Cdc25A, as indicated. (B) AGS and primary tonsil keratinocytes were infected with the AdLacZ or AdBZLF1 vector, and immunoblot analysis was performed 2 days later to quantitate expression of cyclin E and SLBP as indicated.

Article Snippet: Although BZLF1 was previously shown to induce a G 0 /G 1 block in HeLa cells ( 11 , 12 ), the results of the microarray experiment in telomerase-immortalized human keratinocyte cells suggested that BZLF1 is unlikely to induce a G 0 /G 1 cell cycle block in this cell type and instead may be activating cell cycle progression. table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Gene Fold activation E2F-1 16 DP-1 43 Cyclin A 15 Cyclin E 35 Cdc25A 18 Thymidine kinase 5 Thymidylate kinase 21 Open in a separate window a RNA from telomerase-immortalized human keratinocytes, mock infected or infected with AdLacZ or AdBZLF1, was analyzed by microarray analysis using the Affymetrix GeneChip HuGeneFL Array.

Techniques: Expressing, Infection, Plasmid Preparation, Western Blot

Journal:

Article Title: Gene Expression in Asthmatic Airway Smooth Muscle

doi: 10.1513/pats.200705-059VS

Figure Lengend Snippet: GENE EXPRESSION STUDIES USING CULTURED AIRWAY SMOOTH MUSCLE CELLS

Article Snippet: More recently, microarrays have been applied to study asthma therapy by investigating the effects of β-agonists on gene expression in cultured airway smooth muscle cells ( 29 ). table ft1 table-wrap mode="anchored" t5 TABLE 2. caption a7 Author ( Reference No. ) Source of Cells Exposures Method of Gene Expression Profiling Findings Lee and coworkers ( 28 ) Commercially available human airway smooth muscle cells IL-13 (100 ng/ml) Affymetrix Genechip HuGeneFL arrays coding for ∼6,500 human genes 204 Genes induced two-fold, including smooth muscle MHC, phospholipase A2, diacyglycerol kinase δ, and IL-13Rα1 Syed and coworkers ( 30 ) 3 Lung transplant donors IL-13 (10–100 ng/ml) Research Genetics and Incyte Genomics cDNA microarrays coding for 8,159 genes A large number of genes induced 1.5-fold or by FDR analyses (total no. not given), including VCAM1, IL-13Rα2, Tenascin C, and histamine receptor H1 IL-13R130Q * Jarai and coworkers ( 27 ) 2 Patients undergoing lung resection IL-13 † Affymetrix HG-U95Av2 microarrays coding for ∼12,500 genes H1 receptor and tenascin were highly induced by IL-13 TGF-β † IL-1β † McGraw and coworkers ( 29 ) Wild-type and transgenic mice overexpressing β 2 -adrenergic receptor None University of Cincinnati Genomic and Microarray Laboratory array coding for 8,734 cDNAs 319 Genes induced 164 Repressed (including phospholamban) Open in a separate window Definition of abbreviations: FDR = false discovery rate; IL-13Rα1 = IL-13 receptor α1; MHC = myosin heavy chain; RT-PCR = reverse transcription–polymerase chain reaction; TGF = transforming growth factor; VCAM = vascular cell adhesion molecule.

Techniques: Expressing, Cell Culture, Transgenic Assay, Microarray